publication_authors:Hino Y Results

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Microarray (54)

Platform

Affymetrix Mouse Genome 430 2.0 Array (mouse4302) (37)

Affymetrix Human Genome U133 Plus 2.0 Array (hgu133plus2) (17)

Includes Publication

GSE109839

Effect of LSD1 knockdown on differentiating C2C12 myoblasts

Mus musculus
4 Downloadable Samples

Description

Analysis of differentiating LSD1-KD C2C12 myoblasts. We found LSD1 is an important regulator of oxidative phenotypes in skeletal muscle cells.

Publication Title

LSD1 mediates metabolic reprogramming by glucocorticoids during myogenic differentiation.

Sample Metadata Fields

Specimen part, Cell line

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GSE18600

Importance of histone demethylation in adipogenic differentiation and function

Mus musculus
6 Downloadable Samples

Description

This SuperSeries is composed of the SubSeries listed below.

Publication Title

FAD-dependent lysine-specific demethylase-1 regulates cellular energy expenditure.

Sample Metadata Fields

Specimen part

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GSE18598

Differentiating 3T3-L1 adipocytes, introduced with siRNA against aof2 and rfk genes, or treated with tranylcypromine

Mus musculus
4 Downloadable Samples

Description

Adipogenic differentiation and metabolic adaptation are initiated through transcriptional and epigenetic reprogramming. In particular, dynamic changes in histone modifications may play central roles in the rearrangement of gene expression patterns. LSD1 (KDM1) protein, encoded by aof2 gene, is a histone demethylase, which is involved in transcriptional regulation. Since the enzymatic activity of LSD1 is FAD (flavin adenine dinucleotide)-dependent, its effects on gene expression may be influenced by FAD availability.

Publication Title

FAD-dependent lysine-specific demethylase-1 regulates cellular energy expenditure.

Sample Metadata Fields

Specimen part

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GSE18599

Differentiating 3T3-L1 adipocytes, introduced with siRNA against phf21a gene

Mus musculus
2 Downloadable Samples

Description

Adipogenic differentiation and metabolic adaptation are initiated through transcriptional and epigenetic reprogramming. In particular, dynamic changes in histone modifications may play central roles in the rearrangement of gene expression patterns. BHC80 protein, encoded by phf21a gene, is a part of LSD1 histone demethylase complex and is essential for the demethylation activity.

Publication Title

FAD-dependent lysine-specific demethylase-1 regulates cellular energy expenditure.

Sample Metadata Fields

Specimen part

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GSE18052

Analysis of gene expression levels in BBF2H7-/- chondrocytes

Mus musculus
2 Downloadable Samples

Description

BBF2H7 (BBF2 human homolog on chromosome 7), an ER-resident basic leucine zipper transcription factor, is activated in response to ER stress and abundantly expresses in chondrocytes. While BBF2H7 is widely expressed in many tissues and organs, the most intense signals were detected in the proliferating zone of the cartilage. We compared gene expressions in primary cultured chondrocytes prepared from rib cartilage between WT and BBF2H7-/- mice at E18.5. Primary cultured chondrocytes were prepared from E18.5 rib cartilage of WT and BBF2H7-/- mice. Chondrocytes were isolated using 0.2% collagenase D (Roche) after adherent connective tissue was removed by 0.2% trypsin (Sigma) and collagenase pretreatment. Isolated chondrocytes were maintained in -MEM (Gibco) supplemented with 10% FCS and 50 g/mL ascorbic acid. Adenovirus vectors expressing the mouse p60 BBF2H7 (1-377 aa, BBF-N) were constructed with the AdenoX Expression system (Clontech), according to the manufacturers protocol. The cells were infected with adenoviruses 30 h before analysis.

Publication Title

Regulation of endoplasmic reticulum stress response by a BBF2H7-mediated Sec23a pathway is essential for chondrogenesis.

Sample Metadata Fields

Specimen part

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GSE18062

Microarray of RNA from calvaria in WT and OASIS-/- (KO) mice

Mus musculus
2 Downloadable Samples

Description

Investigation of whole genome gene expression level changes in OASIS KO calvaria compared to wild-type calvaria.

Publication Title

Signalling mediated by the endoplasmic reticulum stress transducer OASIS is involved in bone formation.

Sample Metadata Fields

Specimen part

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GSE52794

Gene expression in aortic roots of wild type versus klotho-deficient mice at 6 weeks of age

Mus musculus
6 Downloadable Samples

Description

Klotho-deficient mice develop aortic valve annulus calcification by 6 weeks of age. Understanding the molecular basis by which aortic valve calcification is initiated will help define potential molecular targets which may be inhibited to reduce or prevent aortic valve calcification.

Publication Title

COX2 inhibition reduces aortic valve calcification in vivo.

Sample Metadata Fields

Specimen part

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GSE16522

Effector cells derived from nave or central memory pmel-1 CD8+ T cells

Mus musculus
11 Downloadable Samples

Description

Effector cells for adoptive immunotherapy can be generated by in vitro stimulation of nave or memory subsets of CD8+ T cells. While the characteristics of CD8+ T cell subsets are well defined, the heritable influence of those populations on their effector cell progeny is not well understood. We studied effector cells generated from nave or central memory CD8+ T cells and found that they retained distinct gene expression signatures and developmental programs. Effector cells derived from central memory cells tended to retain their CD62L+ phenotype, but also to acquire KLRG1, an indicator of cellular senescence. In contrast, the effector cell progeny of nave cells displayed reduced terminal differentiation, and, following infusion, they displayed greater expansion, cytokine production, and tumor destruction. These data indicate that effector cells retain a gene expression imprint conferred by their nave or central memory progenitors, and they suggest a strategy for enhancing cancer immunotherapy.